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China, Chinese Huankai Program Controlled Sealing Machine-Enzyme Substrate Coliform Test Onpg Mug-Elite Tray Sealer1 Industrial Products Supplier Manufacturer Details, price list catalog:
China Industrial Products Supplier Manufacturer List Catalog
Laboratory InstrumentsOther Lab InstrumentsTray SealerSealing Machine
Guangdong Huankai Biology Sci.&Tech. Co.,Ltd.
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Product Description Product DescriptionSet of products:HuanKai Enzyme Substrate Coliform Test (ONPG, MUG) - ReagentHuanKai Enzyme Substrate Coliform Test (ONPG, MUG) - Sample VesselHuanKai Enzyme Substrate Coliform Test (ONPG, MUG) - Elite-Tray/51 and Elite-Tray/97HuanKai Enzyme Substrate Coliform Test (ONPG, MUG) - Elite-Tray SealerHuanKai Enzyme Substrate Coliform Test (ONPG, MUG) - UV AnalyzerApplication:Used for the principle of enzyme substrate method to detect water coliform, Escherichia coli and fecal coliform How to usePresence/AbsenceAdd reagent to a 100mL sample, mix well, and incubate at 36°C ± 1°C for 24 hours.Read results:Colorless = negativeYellow = total coliformsYellow/fluorescent = E. coliQuantificationAdd reagent to a 100mL sample and mix well.Pour the sample into Elite-Tray/51 (counts from 1-200) or Elite-Tray/97 (counts from 1-2,419).Seal in Elite-Tray Sealer and place in 36°C ± 1°C incubator for 24 hours.Read results:Yellow wells = total coliformsYellow/fluorescent wells = E. coliCount positive wells and refer to the MPN tablePrincipleThe ScienceThe HuanKai Enzyme Substrate Coliform Test is designed to simultaneously detect total coliforms and E. coli. Two nutrient indicators, ONPG and MUG, are the major sources of carbon in our HuanKai's reagent and can be metabolized by the coliform enzyme β-galactosidase and the E. coli enzyme β-glucuronidase, respectively.As coliforms grow, they use β-galactosidase to metabolize ONPG and change it from colorless to yellow. E. coli use β-glucuronidase to metabolize MUG and create fluorescence. Since most non-coliforms do not have these enzymes, they are unable to grow and interfere. The few non-coliforms that do have these enzymes are selectively suppressed by HuanKai's specifically formulated matrix. Company Information
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