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China, Chinese Separate total RNA spin columns HiPure Total RNA Kit Plant leaves 50-150 mg1 Industrial Products Supplier Manufacturer Details, price list catalog:
China Industrial Products Supplier Manufacturer List Catalog
Chemical ReagentSeparate total RNA spin columnsHiPure Total RNA Kit
Magen Biotechnology (Guangzhou) Co., Ltd.
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Product Description Product DescriptionHiPure Total RNA Kit CAT NOPRODUCT NAMESIZEPRICER401102HiPure Total RNA Kit50 preps$99.00R401103HiPure Total RNA Kit250 preps$448.00The HiPure Total RNA Kit provides fast purification of high-quality RNA from cells, tissues, and yeast using silica-membrane spin columns with a binding capacity of 100μg RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or isopropanol precipitation. RNA purified using the HiPure Total RNA Purification System can be used for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.SpecificationsFeaturesSpecificationsMain FunctionsIsolation total RNA (not include miRNA) from animal tissues, cells and simple plant tissues using one columnApplicationsRT-PCR, qRT-PCR, Northern hybridization, second generation sequencingPurification methodMini spin columnPurification technologySilica technologyProcess methodManual (centrifugation or vacuum)Sample typeAnimal soft tissue, cultured cells, lymphocytes, simple plant tissueSample amountCells: ≤1 x 107Animal tissue: 1-20 mgPlant leaves: 50-150 mgYeast cells: 5 x 106Yield2-100μgElution volume≥50μlTime per run≤15 minutes(1-24 samples)Liquid carrying volume per column800μlBinding yield of column100μgPrincipleHiPure RNA technology simplifies total RNA isolation. Samples are first lysed and then homogenized. Ethanol is added to the lysate to provide ideal binding conditions. The lysate is then loaded onto the HiPure silica membrane and RNA binds to the silica membrane, and all contaminants are efficiently washed away. For certain RNA applications that are sensitive to very small amounts of DNA, the residual amounts of DNA remaining can be removed using a convenient on-column DNase treatment. Pure, concentrated RNA is eluted in water.AdvantagesEfficient removal of DNA - unique genomic DNA removal column without DNase treatmentHigh quality - high purity total RNA can be directly used in various sensitive downstream applicationsFast - several samples can be extracted in 20 minutes by column methodSafe - no phenol chloroform extraction requiredSensitive - RNA can be recovered at the level of PGKit ContentsContentsR401102R401103Purification Times50 Preps250 PrepsHiPure RNA Mini Columns502502ml Collection Tubes50250RTL Lysis Buffer50 ml200 mlRNA Binding Buffer15 ml75 mlBuffer RW150 ml200 mlBuffer RW2*20 ml2 x 50 mlRNase Free Water10 ml30 mlStorage and StabilityThe Kit can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. Detailed Photos Protocol Test Report Company Profile FAQ Q1.What is the Trade Term?A1:Ex-work factory (Guangzhou)Q2. How long is the guarantee (period)?A2:The product can be stored properly for at least 18 months .Q3.Are you a manufacturer ? A3:Yes, we were established in 2012 and our headquarter is in Guangzhou. All products can be provided with OEM packaging, greatly reduce the cost, and can be flexible in technology and other aspects of communication and cooperation.Q4.How long is our Production leading time?A4:Generally our lead time is 2-7 working days after receiving 100% prepayment. If order in bulk , lead time will be longer. Customized bulk quantities are determined by the specific quantity.Q5.What is the Payment term?A5:T/T, Paypal, Alipay, Western Union. 100% payment in advance.Q6.What is the packing details?A6:The outer packaging of general products is cardboard boxes. Low-temperature products will have ice packs places in foam boxes and then packed into cartons.Q7. What support you will have to assemble this extractor?A7: Our extraction instrument is integrated, easy to operate and does not require assembly.Q8.What kind of documents we will provide to you?A8:B/L or AWB number, Commercial Invoice, Packing List, MSDS, COA.Q9. How to apply the free samples?A9: For free samples we have to know your company name, your E-mail, your FedEx account, and the detail address of your company include the phone number. You can contact with us to apply the free samples.Q10. Do you accept the small orders?A10: Yes, we accept the small orders. We will try our best to support our customer's business whether the small trail order.
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