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China, Chinese Fluorescent DNA Sequencing Cloning C Vacuum tumor-specific extracellular DNA fragments Circulating DNA Midi Kit1 Industrial Products Supplier Manufacturer Details, price list catalog:
China Industrial Products Supplier Manufacturer List Catalog
Chemical ReagentHiPure Circulating DNA Midi KitVacuum Method Free-Circulating DNA
Magen Biotechnology (Guangzhou) Co., Ltd.
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Product Description Product DescriptionHiPure Circulating DNA Midi Kit C (Vaccum Protocol) CAT NOPRODUCT NAMESIZEPRICED318201CHiPure Circulating DNA Midi Kit C10 preps$136.00D318202CHiPure Circulating DNA Midi Kit C50 preps$547.00Free-circulating nucleic acids, such as tumor-specific extracellular DNA fragments and mRNAs in the blood or fetal nucleic acids in maternal blood, are present in serum or plasma usually as short fragments, <1000bp (DNA). HiPure Circulating DNA Midi Kit enables efficient purification of these circulating nucleic acids from human plasma, serum, or urine.This product is for research use only.SpecificationsFeaturesSpecificationsMain FunctionsIsolation circulating DNA from 1-5ml plasma, serum, body fluids using vacuum protocolApplicationsqPCR, liquid or solid chip analysis, hybridization and SNP detection, etc.Purification methodMini spin columnPurification technologySilica technologyProcess methodManual (vacuum)Sample typeSerum, plasma and other cell-free fluid samplesSample amount1-5mlElution volume≥50μlTime per run≤60 minutesLiquid carrying volume per column4mlBinding yield of column1mgPrincipleThis product is based on silica Column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer.AdvantagesHigh yield - most optimal process, free DNA (>50bp) can be obtained to the maximum extentHigh concentration - low elution volume, ensuring high nucleic acid concentrationHigh purity - low alcohol binding method, completely removing inhibitor and protein pollutionHigh recovery - DNA can be recovered at thelevel of PG by silica gel column purificationKit ContentsContentsD318201CD318202CPurification Times1050Buffer ACL50 ml250 mlBuffer ACB*60 ml300 mlBuffer DCW1*4.4 ml22 mlBuffer DCW2*5 ml10 mlProteinase K110 ml540 mgProtease Dissolve Buffer10 ml30 mlCarrier RNA110 μg110 μgNuclease Free Water10 ml20 mlHiPure CFDNA Mini Columns10502 ml Collection Tubes20100Extender Tube1050Vac-Connector1050Storage and StabilityProteinase K, Carrier RNA should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used. Experiment Data Detailed Photos Protocol Test Report Company Profile FAQ Q1.What is the Trade Term?A1:Ex-work factory (Guangzhou)Q2. How long is the guarantee (period)?A2:The product can be stored properly for at least 18 months .Q3.Are you a manufacturer ? A3:Yes, we were established in 2012 and our headquarter is in Guangzhou. All products can be provided with OEM packaging, greatly reduce the cost, and can be flexible in technology and other aspects of communication and cooperation.Q4.How long is our Production leading time?A4:Generally our lead time is 2-7 working days after receiving 100% prepayment. If order in bulk , lead time will be longer. Customized bulk quantities are determined by the specific quantity.Q5.What is the Payment term?A5:T/T, Paypal, Alipay, Western Union. 100% payment in advance.Q6.What is the packing details?A6:The outer packaging of general products is cardboard boxes. Low-temperature products will have ice packs places in foam boxes and then packed into cartons.Q7. What support you will have to assemble this extractor?A7: Our extraction instrument is integrated, easy to operate and does not require assembly.Q8.What kind of documents we will provide to you?A8:B/L or AWB number, Commercial Invoice, Packing List, MSDS, COA.Q9. How to apply the free samples?A9: For free samples we have to know your company name, your E-mail, your FedEx account, and the detail address of your company include the phone number. You can contact with us to apply the free samples.Q10. Do you accept the small orders?A10: Yes, we accept the small orders. We will try our best to support our customer's business whether the small trail order.
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